Home > Cancer - Information & Herbal Health! > Agaricus Blazei Murill Mushroom (ABM) > ABM Mushroom Research > ABM MUSHROOM RESEARCH - JOURNAL REFERENCE ITEM 8: ABM MUSHROOM RESEARCH - JOURNAL REFERENCE ITEM 7: ABM MUSHROOM RESEARCH - JOURNAL REFERENCE ITEM 1: Back to Top ___________________________________________ ABM MUSHROOM RESEARCH - JOURNAL REFERENCE ITEM 8: TITLE: Anti-tumor Activity and Some Properties of Water-insoluble Hetero-glycans from Himematsutake, the Fruiting Body of Agaricus blazei Murill. AUTHORS: Mizuno T, Inagaki R, Kanao T, Hagiwara T, Nakamura T, Ito H, Shimura K, Sumiya T, Asakura A JOURNAL: Agricultural and Biological Chemistry; 54(11):2889-2896 1990 ADDRESS: Faculty of Agriculture, Shizuoku Univ., Ohya, Shizuoku 422, Japan ABSTRACT: After extraction of a hot-water-soluble polysaccharide (FI) from the fruiting bodies of Himematsutake (Agaricus blazei Murill), water-insoluble polysaccharides were obtained by successive extraction with 1% ammonium oxalate solution (FII), 5% sodium hydroxide solution (FIII and FIV), 20% sodium hydroxide solution (FV), and 5% lithium chloride-dimethylacetamide solution (FVI) in that order. These water-insoluble fractions were futher fractionated by ethanol precipitation, gel-filtration, etc. Polysaccharides, polysaccharide-protein complexes, and chitin substances thus obtained were assayed for their anti-tumor activities using the Sarcome 180/mice i.p. o.p. method. The heteroglycan-protein complexes, FII-a,-b,-c, obtained from FII had weak antitumor activities. A remarkable antitumor activity was found in a lycoprotein, FIII-2-b, fractionated and purified from FIII. The polysaccharide portion of this polysaccharide-protein complex (polysaccharide, 50.2% and protein, 43.3% each on a weight basis) consisted of (1--6)-beta-D-glucan, and its protein portion was rich in Asx, Glx, Ala, Leu, and Pro. A high antitumor activity was found in a xyloglucan-protein complex, FIV-2-b, fractionated and purified from FIV. Antitumor activity was found also in a glucoxylan, FV-2-a, obtained from FV. No significant antitumor activity was found in a chitin substance, FVI. Back to Top ___________________________________________ ABM MUSHROOM RESEARCH - JOURNAL REFERENCE ITEM 7: TITLE: Antitumor Activity and Some Properties of Water-soluble Polysaccharides from Himematsutake, the Fruiting Body of Agaricus blazei Murill. AUTHORS: Mizuno T, Hagiwara T, Nakamura T, Ito H, Shimura K, Sumiya T, Asakura A JOURNAL: Agricultural and Biological Chemistry; 54(11):2897-2906 1990 ADDRESS: Faculty of Agriculture, Shizuoku Univ., Ohya, Shizuoku 422, Japan ABSTRACT: Polysaccharides extracted from Himematsutake, the fruiting body of Agaricus blazei Murill with hot water were fractionated and purified by ethanol precipitation, ion-exchange chromatography, gel-filtration, affinity chromatography, etc. A total of 17 polysaccharide samples thus obtained were given an antitumor activity test (Sarcoma 180/mice i.p. p.o. method) and traces of their activities through the fractionation and purification processes were found. FI0-a-beta, FA-1-a-alpha, FA-1-a-beta, and FA-2-b-beta, were obtained as water soluble polysaccharides fractions having great antitumor activities. Analyses of physico-chemical properties and IR- and NMR-spectra of the these active fractions showed that their main components were: FI0-a-beta, (1--6)-; (1--3)-beta-D-glucan; FA-1-a-alpha, acidic (1--6)-; (1--4)-alpha-D-glucan; FA-1-a-beta, acidic (1--6)-; (1--3)-alpha-D-glucan; and FA-2-b-beta, acidic RNA-protein complex. Back to Top ___________________________________________ ABM MUSHROOM RESEARCH - JOURNAL REFERENCE ITEM 1: TITLE: Selective Tumoricidal Effect of Soluble Proteoglucan Extracted from the Basidiomycete, Agaricus blazei Murill, Mediated Via Natural Killer Cell Activation and Apoptosis. AUTHORS: Fujimiya Y, Suzuki Y, Oshiman K, Kobori H, Moriguchi K, Nakashima H, Matumoto Y, Takahara S, Ebina T, Katakura R JOURNAL: Cancer Immunol Immunother; 46(3):147-59 1998 ADDRESS: Division of Immunology, Miyagi Cancer Center Research Institute, Natori, Japan. ABSTRACT: We have isolated a novel type of natural tumoricidal product from the basidiomycete strain, Agaricus blazei Murill. Using the double-grafted tumor system in Balb/c mice, treatment of the primary tumor with an acid-treated fraction (ATF) obtained from the fruit bodies resulted in infiltration of the distant tumor by natural killer (NK) cells with marked tumoricidal activity. As shown by electrophoresis and DNA fragmentation assay, the ATF also directly inhibited tumor cell growth in vitro by inducing apoptotic processing; this apoptotic effect was also demonstrated by increased expression of the Apo2.7 antigen on the mitochondrial membranes of tumor cells, as shown by flow-cytometric analysis.The ATF had no effect on normal mouse splenic or interleukin-2-treated splenic mononuclear cells, indicating that it is selectively cytotoxic for the tumor cells. Cell-cycle analysis demonstrated that ATF induced the loss of S phase in MethA tumor cells, but did not affect normal splenic mononuclear cells, which were mainly in the G0G1 phase. Various chromatofocussing purification steps and NMR analysis showed the tumoricidal activity to be chiefly present in fractions containing (1-->4)-alpha-D-glucan and (1-->6)-beta-D-glucan, present in a ratio of approximately 1:2 in the ATF (molecular mass 170 kDa), while the final purified fraction, HM3-G (molecular mass 380 kDa), with the highest tumoricidal activity, consisted of more than 90% glucose, the main component being (1-->4)-alpha-D-glucan with (1-->6)-beta branching, in the ratio of approximately 4:1. Back to Top ___________________________________________ The ABM Mushroom is one of the Finest Health Promoting Products Available. You Can Buy It With Confidence From Insensual.com, Which Has Been Rated The TOP SERVICE Award For Online Customer Satisfaction.